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Pacific Blue? Annexin V Apoptosis Detection Kit with 7-AAD   

詳細描述

Pacific Blue? Annexin V Apoptosis Detection Kit with 7-AAD
Reactivity: All mammalian species
Storage & Handling: Store between 2°C and 8°C. Do not freeze.

Caution: 7-AAD is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, and eye/face protection in order to avoid contact with skin and eyes.
Application:

FC - Quality tested

Application Notes:

Materials Provided:
0.5 ml of Pacific Blue? Annexin V
0.5 ml of 7-AAD Viability Staining Solution
50 ml of Annexin V Binding Buffer

Materials Not Included:
Cell Staining Buffer

Recommended Usage:

Staining Procedure:
1.25-1.0 x 107 cells/ml.
2. Transfer 100 μl of cell suspension in a 5 ml test tube.
3. Add 5 μl of Pacific Blue? Annexin V.
4. Add 5 μl of 7-AAD Viability Staining Solution.
5. Gently vortex the cells and incubate for 15 min at room temperature (25°C) in the dark.
6. Add 400 μl of Annexin V Binding Buffer to each tube. Analyze by flow cytometry with proper machine settings.

Application References:

1. Maciel E, et al. 2014. Arch Biochem Biophys. 548:38.?PubMed

Human T-cell leukemia cell line,

Human T-cell leukemia cell line, Jurkat, treated (top) or non-treated (bottom) with LEAF? purified anti-human CD95 (clone EOS9.1) for 4 hours, then stained with Pacific Blue? Annexin V Apoptosis Detection Kit with 7-AAD.




Description:

Pacific Blue? Annexin V Apoptosis Detection Kit with 7-AAD has been specifically designed for the identification of apoptotic and necrotic cells.

Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer is recommended for use with Annexin V staining. Annexin V binding alone cannot differentiate between apoptotic and necrotic cells. To help distinguish between the necrotic and apoptotic cells we recommend use of our 7-amino-actinomycin D (7-AAD) solution. Early apoptotic cells will exclude 7-AAD, while late stage apoptotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA.

7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. When excited by 488 laser light, 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).

Other Names: Annexin A5 Apoptosis Detection Kit

  • 目錄號:3804630
  • 品牌:Sony Biotechnology
  • 規格:100 tests
  • 目錄價:¥4110.00
  • 市場價格:¥2671.50
  • 會員價格:¥2548.20
  • 數量:

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